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psbZ-trnG-GCC

LSC · 35103-35391 · 289 bp

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Pi 0.0106

MarkerSeek score 53.80

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 289
variable_sites: 6
pi: 0.0106
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TGTCGTATTTGCTTCTCCGG	AATCGAACCCGCATCTTCTC	50.0	50.0	58.0	58.1	462-466 bp	1.0	81.0
psbZ-trnG-GCC_p2	TGTCGTATTTGCTTCTCCGG	GAATCGAACCCGCATCTTCT	50.0	50.0	58.0	58.1	463-467 bp	1.0	81.0
psbZ-trnG-GCC_p3	TGTCGTATTTGCTTCTCCGG	AAAAGGACCTGATCTTGGGC	50.0	50.0	58.0	57.8	492-496 bp	1.0	79.6
psbZ-trnG-GCC_p4	GTCGTATTTGCTTCTCCGGA	AATCGAACCCGCATCTTCTC	50.0	50.0	57.7	58.1	461-465 bp	1.0	78.8
psbZ-trnG-GCC_p5	GTCGTATTTGCTTCTCCGGA	GAATCGAACCCGCATCTTCT	50.0	50.0	57.7	58.1	462-466 bp	1.0	78.8