Hotspot

trnS-GCU-trnG-UCC

LSC · 7715-8386 · 672 bp

Back to Dicliptera

Pi 0.00119

MarkerSeek score 48.40

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 672
variable_sites: 2
pi: 0.00119
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	741 bp	1.0	51.9
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	740 bp	1.0	51.9
trnS-GCU-trnG-UCC_p3	CAATCCGACGCTTTAGTCCA	GGAATATGACCGAAAGAACCT	50.0	42.9	58.0	55.4	783 bp	1.0	51.2
trnS-GCU-trnG-UCC_p4	AATCCGACGCTTTAGTCCAC	GGAATATGACCGAAAGAACCT	50.0	42.9	58.0	55.4	782 bp	1.0	51.2
trnS-GCU-trnG-UCC_p5	ACGGAAAGAGAGGGATTCGA	ACGAATCACACTTTTACCACT	50.0	38.1	58.2	55.5	795 bp	1.0	50.7