Hotspot

rps16-trnQ-UUG

LSC · 5659-6371 · 713 bp

Back to Dicliptera

Pi 0.00112
MarkerSeek score 48.40
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
713
variable_sites
2
pi
0.00112
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 GCTTTCTGCCACATCGTTTC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 774-775 bp 1.0 79.4
rps16-trnQ-UUG_p2 AACGGATTCTGCCCTTCAAG GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 803-804 bp 1.0 78.2
rps16-trnQ-UUG_p3 GCTTTCTGCCACATCGTTTC AAAATTGAAATGGGGCGTGG 50.0 45.0 58.0 57.5 832-833 bp 1.0 76.2
rps16-trnQ-UUG_p4 CAACGGATTCTGCCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.5 58.1 804-805 bp 1.0 75.9
rps16-trnQ-UUG_p5 AACGGATTCTGCCCTTCAAG AAAATTGAAATGGGGCGTGG 50.0 45.0 58.2 57.5 861-862 bp 1.0 75.0