Hotspot

trnH-GUG-psbA

LSC · 74-364 · 291 bp

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Pi 0.00275
MarkerSeek score 31.80
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
291
variable_sites
2
pi
0.00275
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
31.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG CCCTCTAGATTTAGCTGCTAGC 55.0 50.0 58.1 57.8 355 bp 1.0 61.9
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA CCCTCTAGATTTAGCTGCTAGC 50.0 50.0 57.9 57.8 369 bp 1.0 61.9
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC CCCTCTAGATTTAGCTGCTAGC 50.0 50.0 57.9 57.8 368 bp 1.0 61.9
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC CCCTCTAGATTTAGCTGCTAGC 50.0 50.0 57.9 57.8 370 bp 1.0 61.9
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG TCGTGCTAATCTTGGTATGGA 55.0 42.9 58.1 56.5 406 bp 1.0 59.7