Hotspot

infA-rps8

LSC · 79215-79403 · 189 bp

Back to Dichocarpum

Pi 0.0353

MarkerSeek score 60.80

Primer available yes

Label infA-rps8

Feature Metadata

label_name: infA-rps8
feature_type: igs
region: LSC
length_bp: 189
variable_sites: 10
pi: 0.0353
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rps8_p1	CCACCCGACCCTGTATATCT	AAAACGTATCAGTCGACCCG	55.0	50.0	58.0	58.0	1022-1047 bp	1.0	84.7
infA-rps8_p2	CCACCCGACCCTGTATATCT	TTGGTTTCAACTCTGCGACA	55.0	45.0	58.0	58.0	1078-1103 bp	1.0	84.3
infA-rps8_p3	AAATTCTGCGTTCCCTGGTT	AAAACGTATCAGTCGACCCG	45.0	50.0	58.0	58.0	509-510 bp	1.0	83.8
infA-rps8_p4	AAATTCTGCGTTCCCTGGTT	TTGGTTTCAACTCTGCGACA	45.0	45.0	58.0	58.0	565-566 bp	1.0	83.2
infA-rps8_p5	ACCAATGCGTCCATTCCTAC	AAAACGTATCAGTCGACCCG	50.0	50.0	58.0	58.0	947 bp	0.666667	68.0