Hotspot

psbT-psbN

LSC · 73508-73601 · 94 bp

Back to Dichocarpum

Pi 0.0507

MarkerSeek score 71.90

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 94
variable_sites: 7
pi: 0.0507
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	CCCAAATGAACAGGTGTGGA	ATATTGCGTGCTCCGGATAC	50.0	50.0	58.0	57.9	938-940 bp	1.0	81.2
psbT-psbN_p2	TCCCAAATGAACAGGTGTGG	ATATTGCGTGCTCCGGATAC	50.0	50.0	58.0	57.9	939-941 bp	1.0	81.2
psbT-psbN_p3	CCCAAATGAACAGGTGTGGA	CGAAGCAGAACCCATCTCTC	50.0	55.0	58.0	58.1	902-904 bp	0.666667	66.8
psbT-psbN_p4	TCCCAAATGAACAGGTGTGG	CGAAGCAGAACCCATCTCTC	50.0	55.0	58.0	58.1	903-905 bp	0.666667	66.8
psbT-psbN_p5	ATTGTCAGAGCCGATGTTCC	CGAAGCAGAACCCATCTCTC	50.0	55.0	58.0	58.1	1439-1440 bp	0.666667	66.6