Hotspot

atpA-atpF

LSC · 10607-10672 · 66 bp

Back to Dichocarpum

Pi 0.0404

MarkerSeek score 72.60

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 66
variable_sites: 4
pi: 0.0404
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	CTGCTCAATACGTTCACGGA	TGTTTGGGGCCATGAAAGAA	50.0	45.0	58.0	57.9	155 bp	1.0	81.2
atpA-atpF_p2	CCTACTTGAAGTACGGTGCC	TGTTTGGGGCCATGAAAGAA	55.0	45.0	58.0	57.9	205 bp	1.0	80.8
atpA-atpF_p3	CTGCTCAATACGTTCACGGA	GGTATGTTTGGGGCCATGAA	50.0	50.0	58.0	58.1	159 bp	1.0	80.8
atpA-atpF_p4	CCTACTTGAAGTACGGTGCC	GGTATGTTTGGGGCCATGAA	55.0	50.0	58.0	58.1	209 bp	1.0	80.4
atpA-atpF_p5	CCTACTTGAAGTACGGTGCC	ATGTTTGGGGCCATGAAAGA	55.0	45.0	58.0	57.7	206 bp	1.0	78.9