Hotspot

trnG-UCC-trnR-UCU

LSC · 8676-8872 · 197 bp

Back to Dichocarpum

Pi 0.04

MarkerSeek score 70.50

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 197
variable_sites: 10
pi: 0.04
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	274-278 bp	1.0	70.2
trnG-UCC-trnR-UCU_p2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	55.0	47.6	58.1	56.9	277-281 bp	1.0	70.0
trnG-UCC-trnR-UCU_p3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	280-284 bp	1.0	69.5
trnG-UCC-trnR-UCU_p4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	55.0	45.5	58.1	57.9	283-287 bp	1.0	69.3
trnG-UCC-trnR-UCU_p5	ATGATGAATTGGCGTCGACT	AGAAGACCTCTGTCCTATCCA	45.0	47.6	57.7	56.9	303-307 bp	1.0	67.7