Hotspot

trnK-UUU-rps16

LSC · 4014-4699 · 686 bp

Back to Dichocarpum

Pi 0.0392

MarkerSeek score 76.90

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 686
variable_sites: 37
pi: 0.0392
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	CCTTGAAAAAGGCGCTCAAC	50.0	50.0	58.2	58.2	769-823 bp	1.0	84.5
trnK-UUU-rps16_p2	AAAGCCGAGTACTCTACCGT	CTTGAAAAAGGCGCTCAACC	50.0	50.0	58.2	58.2	768-822 bp	1.0	84.5
trnK-UUU-rps16_p3	AAGCCGAGTACTCTACCGTT	CCTTGAAAAAGGCGCTCAAC	50.0	50.0	58.2	58.2	768-822 bp	1.0	84.5
trnK-UUU-rps16_p4	AAGCCGAGTACTCTACCGTT	CTTGAAAAAGGCGCTCAACC	50.0	50.0	58.2	58.2	767-821 bp	1.0	84.5
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	TGAAAAAGGCGCTCAACCTA	50.0	45.0	58.2	57.7	765-819 bp	1.0	84.2