Hotspot

ndhK

LSC · 47919-48668 · 750 bp

Back to Dichanthium

Pi 0.00268

MarkerSeek score 60.80

Primer available yes

Label ndhK

Feature Metadata

label_name: ndhK
feature_type: gene
region: LSC
length_bp: 750
variable_sites: 3
pi: 0.00268
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK_p1	TCTGCACAAAAGAACCCCTC	TCTCTACCCTTGGGCAATGA	50.0	50.0	58.0	58.0	890-902 bp	1.0	80.1
ndhK_p2	TCTGCACAAAAGAACCCCTC	CGACGTATTGGGTGTATCCG	50.0	55.0	58.0	58.2	866-878 bp	1.0	78.5
ndhK_p3	GATAACCAACCCTGCTGCAT	TCTCTACCCTTGGGCAATGA	50.0	50.0	58.2	58.0	988-1000 bp	1.0	78.3
ndhK_p4	ATAACCAACCCTGCTGCATT	TCTCTACCCTTGGGCAATGA	45.0	50.0	57.8	58.0	987-999 bp	1.0	78.3
ndhK_p5	TCTGCACAAAAGAACCCCTC	GGGCAATGAGTTTCGACGTA	50.0	50.0	58.0	58.3	879-891 bp	1.0	78.1