Hotspot

ccsA

SSC · 105673-106641 · 969 bp

Back to Dichanthium

Pi 0.00621

MarkerSeek score 60.20

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 9
pi: 0.00621
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	CTCAAGCATCTCGGTTCGAA	TCCGTTGATAGGGTAGAGGT	50.0	50.0	58.0	56.9	1151-1284 bp	1.0	71.6
ccsA_p2	AAGCATCTCGGTTCGAATCC	TCCGTTGATAGGGTAGAGGT	50.0	50.0	58.1	56.9	1148-1281 bp	1.0	71.2
ccsA_p3	CTCAAGCATCTCGGTTCGAA	TCCGTTGATAGGGTAGAGGTT	50.0	47.6	58.0	57.6	1151-1284 bp	1.0	69.6
ccsA_p4	AAGCATCTCGGTTCGAATCC	TCCGTTGATAGGGTAGAGGTT	50.0	47.6	58.1	57.6	1148-1281 bp	1.0	69.2
ccsA_p5	CTCAAGCATCTCGGTTCGAA	ATCCGTTGATAGGGTAGAGGT	50.0	47.6	58.0	57.4	1152-1285 bp	1.0	67.8