Hotspot

psaC-ndhE

SSC · 108533-108999 · 467 bp

Back to Dichanthium

Pi 0.0143

MarkerSeek score 67.50

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 467
variable_sites: 10
pi: 0.0143
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	TACATCTGTTGGGCAAGCTC	TGTTATAGCCCTTGCAGCTG	50.0	50.0	57.9	58.0	644-654 bp	1.0	81.2
psaC-ndhE_p2	GGCAAGCTCGTACACATTGA	TGTTATAGCCCTTGCAGCTG	50.0	50.0	58.6	58.0	633-643 bp	1.0	77.7
psaC-ndhE_p3	GCAAGCTCGTACACATTGAG	TGTTATAGCCCTTGCAGCTG	50.0	50.0	57.2	58.0	632-642 bp	1.0	75.8
psaC-ndhE_p4	ATACATCTGTTGGGCAAGCT	TGTTATAGCCCTTGCAGCTG	45.0	50.0	57.2	58.0	645-655 bp	1.0	75.6
psaC-ndhE_p5	TACATCTGTTGGGCAAGCTC	TTGTTATAGCCCTTGCAGCT	50.0	45.0	57.9	57.2	645-655 bp	1.0	75.1