Hotspot

trnP-TGG-psaJ

LSC · 63028-63412 · 385 bp

Back to Dichanthium

Pi 0.0182

MarkerSeek score 68.30

Primer available yes

Label trnP-TGG-psaJ

Feature Metadata

label_name: trnP-TGG-psaJ
feature_type: igs
region: LSC
length_bp: 385
variable_sites: 8
pi: 0.0182
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-TGG-psaJ_p1	TCCCCACTACTGCCCATAAA	AAACCTGCTAAAGCCCCAAA	50.0	45.0	58.0	57.9	562-613 bp	1.0	81.6
trnP-TGG-psaJ_p2	AAAACAAACGCGCTACCAAG	AAACCTGCTAAAGCCCCAAA	45.0	45.0	57.9	57.9	485-536 bp	1.0	81.3
trnP-TGG-psaJ_p3	CAAAACAAACGCGCTACCAA	AAACCTGCTAAAGCCCCAAA	45.0	45.0	58.2	57.9	486-537 bp	1.0	80.9
trnP-TGG-psaJ_p4	TTGTACCCAAAACAAACGCG	AAACCTGCTAAAGCCCCAAA	45.0	45.0	57.8	57.9	493-544 bp	1.0	80.7
trnP-TGG-psaJ_p5	CCCCACTACTGCCCATAAAG	AAACCTGCTAAAGCCCCAAA	55.0	45.0	57.7	57.9	561-612 bp	1.0	79.1