Hotspot

trnD-GTC-psbM

LSC · 15379-16102 · 724 bp

Back to Dichanthium

Pi 0.00184

MarkerSeek score 60.40

Primer available yes

Label trnD-GTC-psbM

Feature Metadata

label_name: trnD-GTC-psbM
feature_type: igs
region: LSC
length_bp: 724
variable_sites: 2
pi: 0.00184
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GTC-psbM_p1	AATAGGCATGCCATACACCC	GGCAGTAGGAACTAGAATGAACA	50.0	43.5	57.7	57.9	882-1215 bp	1.0	53.3
trnD-GTC-psbM_p2	TTCAATTGGTCAGAGCACCG	GGCAGTAGGAACTAGAATGAACA	50.0	43.5	58.5	57.9	848-1181 bp	1.0	51.9
trnD-GTC-psbM_p3	AATAGGCATGCCATACACCC	AGGCAGTAGGAACTAGAATGAAC	50.0	43.5	57.7	57.7	883-1216 bp	1.0	51.3
trnD-GTC-psbM_p4	AATAGGCATGCCATACACCC	AGGCAGTAGGAACTAGAATGA	50.0	42.9	57.7	55.5	883-1216 bp	1.0	50.0
trnD-GTC-psbM_p5	AATAGGCATGCCATACACCC	TGCGAGAATATTGACTTCCA	50.0	40.0	57.7	54.5	843-1176 bp	1.0	49.9