Hotspot

trnG-GCC-trnfM-CAT

LSC · 12255-12577 · 323 bp

Back to Dichanthium

Pi 0.0165

MarkerSeek score 63.80

Primer available yes

Label trnG-GCC-trnfM-CAT

Feature Metadata

label_name: trnG-GCC-trnfM-CAT
feature_type: igs
region: LSC
length_bp: 323
variable_sites: 7
pi: 0.0165
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAT_p1	AATATAGGCCGTAGCAAGCG	CGAGGCTCATAACCTTGAGG	50.0	55.0	57.9	57.8	473-543 bp	1.0	78.9
trnG-GCC-trnfM-CAT_p2	AATATAGGCCGTAGCAAGCG	CAGTTTTCGGCCCTACAGAG	50.0	55.0	57.9	58.3	518-588 bp	1.0	78.5
trnG-GCC-trnfM-CAT_p3	AATATAGGCCGTAGCAAGCG	CAGTTTGGTAGCTCACGAGG	50.0	55.0	57.9	58.3	488-558 bp	1.0	78.4
trnG-GCC-trnfM-CAT_p4	AATATAGGCCGTAGCAAGCG	CTACAGAGCGGAGTAGAGCA	50.0	55.0	57.9	58.3	506-576 bp	1.0	78.0
trnG-GCC-trnfM-CAT_p5	AATATAGGCCGTAGCAAGCG	TACAGAGCGGAGTAGAGCAG	50.0	55.0	57.9	58.3	505-575 bp	1.0	78.0