Hotspot

rps16-trnQ-TTG

LSC · 5587-6676 · 1090 bp

Back to Dichanthium

Pi 0.035

MarkerSeek score 77.10

Primer available yes

Label rps16-trnQ-TTG

Feature Metadata

label_name: rps16-trnQ-TTG
feature_type: igs
region: LSC
length_bp: 1090
variable_sites: 54
pi: 0.035
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-TTG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1136-1163 bp	1.0	85.8
rps16-trnQ-TTG_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1147-1174 bp	1.0	85.3
rps16-trnQ-TTG_p3	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	45.0	55.0	58.1	57.8	1140-1167 bp	1.0	84.7
rps16-trnQ-TTG_p4	TCATGTCCTTCAAGTCGCAC	CTCGGAGGTTCGAATCCTTC	50.0	55.0	58.2	57.8	1151-1178 bp	1.0	84.1
rps16-trnQ-TTG_p5	AAGTCGCACGTTGCTTTCTA	TACTCGGAGGTTCGAATCCT	45.0	50.0	58.1	57.6	1142-1169 bp	1.0	83.0