Hotspot

matK-rps16

LSC · 3215-4495 · 1281 bp

Back to Dichanthium

Pi 0.00366

MarkerSeek score 61.70

Primer available yes

Label matK-rps16

Feature Metadata

label_name: matK-rps16
feature_type: igs
region: LSC
length_bp: 1281
variable_sites: 7
pi: 0.00366
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-rps16_p1	ACGAAGTAGTGTTGACGAGA	GGTGCTCAACCTACAAGAACA	45.0	47.6	56.3	58.2	1418-1435 bp	1.0	57.8
matK-rps16_p2	TGAAAGAGAAGTGGGTAGACG	GGTGCTCAACCTACAAGAACA	47.6	47.6	57.1	58.2	1436-1453 bp	1.0	56.1
matK-rps16_p3	AGAGAAGTGGGTAGACGAAG	GGTGCTCAACCTACAAGAACA	50.0	47.6	56.0	58.2	1432-1449 bp	1.0	55.7
matK-rps16_p4	TTCCTGAAAGAGAAGTGGGT	GGTGCTCAACCTACAAGAACA	45.0	47.6	56.0	58.2	1440-1457 bp	1.0	55.4
matK-rps16_p5	TCCTGAAAGAGAAGTGGGTAGA	GGTGCTCAACCTACAAGAACA	45.5	47.6	57.9	58.2	1439-1456 bp	1.0	54.6