Hotspot

rpl16

LSC · 79181-80683 · 1503 bp

Back to Dichanthelium

Pi 0.00226

MarkerSeek score 52.20

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1503
variable_sites: 10
pi: 0.00226
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCCAGAAAGAAAAACCGGGG	GAAGAAATTATACCTCTTTCTATAAGA	50.0	25.9	58.0	53.0	1616-1622 bp	1.0	40.8
rpl16_p2	TCCAGAAAGAAAAACCGGGG	AGAAGAAATTATACCTCTTTCTATAAG	50.0	25.9	58.0	53.0	1617-1623 bp	1.0	40.8
rpl16_p3	CAGAAAGAAAAACCGGGGGT	GAAGAAATTATACCTCTTTCTATAAGA	50.0	25.9	58.3	53.0	1614-1620 bp	1.0	40.8
rpl16_p4	CAGAAAGAAAAACCGGGGGT	AGAAGAAATTATACCTCTTTCTATAAG	50.0	25.9	58.3	53.0	1615-1621 bp	1.0	40.8
rpl16_p5	CCAGAAAGAAAAACCGGGGG	GAAGAAATTATACCTCTTTCTATAAGA	55.0	25.9	59.0	53.0	1615-1621 bp	1.0	40.8