Hotspot

ycf4-cemA

LSC · 59935-60297 · 363 bp

Back to Dichanthelium

Pi 0.00349

MarkerSeek score 51.40

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 363
variable_sites: 3
pi: 0.00349
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TGCGCGTACCAATTGAAGTA	AGAGAAAGAGACTCCCCAGG	45.0	55.0	57.9	57.8	463-469 bp	1.0	78.8
ycf4-cemA_p2	CTTGCGCGTACCAATTGAAG	AGAGAAAGAGACTCCCCAGG	50.0	55.0	58.1	57.8	465-471 bp	1.0	78.5
ycf4-cemA_p3	ACAAAAAGCTGCCGAATTGG	AGAGAAAGAGACTCCCCAGG	45.0	55.0	57.8	57.8	492-498 bp	1.0	78.1
ycf4-cemA_p4	TTTCTTGCGCGTACCAATTG	AGAGAAAGAGACTCCCCAGG	45.0	55.0	57.7	57.8	468-474 bp	1.0	76.8
ycf4-cemA_p5	CCTATTTCTTGCGCGTACCA	AGAGAAAGAGACTCCCCAGG	50.0	55.0	58.4	57.8	472-478 bp	1.0	76.6