Hotspot

rbcL-psaI

LSC · 57902-58901 · 1000 bp

Back to Dichanthelium

Pi 0.00632

MarkerSeek score 47.20

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 1000
variable_sites: 16
pi: 0.00632
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 47.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	ATCAAATTTGAGTTCGCGCC	GAGGGTAAGTTAAAATCTGTCA	45.0	36.4	57.7	53.5	1030-1062 bp	1.0	43.8
rbcL-psaI_p2	ATCAAATTTGAGTTCGCGCC	GAGGGTAAGTTAAAATCTGTCAT	45.0	34.8	57.7	54.1	1030-1062 bp	1.0	43.8
rbcL-psaI_p3	CGCAGCTTGTGAAGTATGGA	GAGGGTAAGTTAAAATCTGTCA	50.0	36.4	58.3	53.5	1055-1087 bp	1.0	43.7
rbcL-psaI_p4	AGCTTGCAAATGGAGTCCTG	GAGGGTAAGTTAAAATCTGTCA	50.0	36.4	58.5	53.5	1082-1114 bp	1.0	43.6
rbcL-psaI_p5	GCTTGCAAATGGAGTCCTGA	GAGGGTAAGTTAAAATCTGTCA	50.0	36.4	58.5	53.5	1081-1113 bp	1.0	43.6