Hotspot

ndhC-trnV-UAC

LSC · 51807-52736 · 930 bp

Back to Dichanthelium

Pi 0.00523

MarkerSeek score 54.60

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 930
variable_sites: 12
pi: 0.00523
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CTAACCGGGGCTAAAAGTCC	GGTTCGAATCCGTATAGCCC	55.0	55.0	58.0	57.9	1053-1087 bp	1.0	81.4
ndhC-trnV-UAC_p2	CTAACCGGGGCTAAAAGTCC	CGGTTCGAATCCGTATAGCC	55.0	55.0	58.0	58.3	1054-1088 bp	1.0	79.5
ndhC-trnV-UAC_p3	TAACCGGGGCTAAAAGTCCT	GGTTCGAATCCGTATAGCCC	50.0	55.0	58.3	57.9	1052-1086 bp	1.0	78.9
ndhC-trnV-UAC_p4	CACTAACCGGGGCTAAAAGT	GGTTCGAATCCGTATAGCCC	50.0	55.0	57.5	57.9	1055-1089 bp	1.0	77.8
ndhC-trnV-UAC_p5	TAACCGGGGCTAAAAGTCCT	CGGTTCGAATCCGTATAGCC	50.0	55.0	58.3	58.3	1053-1087 bp	1.0	77.1