Hotspot

petN-trnC-GCA

LSC · 18802-19732 · 931 bp

Back to Dichanthelium

Pi 0.00294

MarkerSeek score 51.20

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 931
variable_sites: 7
pi: 0.00294
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	AGCCCAAGCGAGACTTACTA	CTGCAAATCCTTTACCCCCA	50.0	50.0	58.2	57.8	993-999 bp	1.0	78.4
petN-trnC-GCA_p2	AGCCCAAGCGAGACTTACTA	TGCAAATCCTTTACCCCCAG	50.0	50.0	58.2	57.8	992-998 bp	1.0	78.4
petN-trnC-GCA_p3	AGCCCAAGCGAGACTTACTA	ACTGCAAATCCTTTACCCCC	50.0	50.0	58.2	57.8	994-1000 bp	1.0	78.4
petN-trnC-GCA_p4	AGCCCAAGCGAGACTTACTA	GCAAATCCTTTACCCCCAGT	50.0	50.0	58.2	57.8	991-997 bp	1.0	78.4
petN-trnC-GCA_p5	CCACTCCTCCCCCATACTAC	TGCAAATCCTTTACCCCCAG	60.0	50.0	58.3	57.8	1048-1054 bp	1.0	77.3