Hotspot

trnT-GGU-trnE-UUC

LSC · 16027-16487 · 461 bp

Back to Dichanthelium

Pi 0.00261

MarkerSeek score 51.40

Primer available yes

Label trnT-GGU-trnE-UUC

Feature Metadata

label_name: trnT-GGU-trnE-UUC
feature_type: igs
region: LSC
length_bp: 461
variable_sites: 3
pi: 0.00261
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnE-UUC_p1	AGGGCTTTTCACTTAGTGGT	GCTGCCTCCTTGAAAGAGAG	45.0	55.0	56.7	58.3	571-578 bp	1.0	69.2
trnT-GGU-trnE-UUC_p2	AGGGCTTTTCACTTAGTGGT	TGTCCTGAACCACTAGACGA	45.0	50.0	56.7	57.7	550-557 bp	1.0	69.1
trnT-GGU-trnE-UUC_p3	AGGCATAAGTCATCGGTTCA	GCTGCCTCCTTGAAAGAGAG	45.0	55.0	56.6	58.3	601-608 bp	1.0	68.9
trnT-GGU-trnE-UUC_p4	AGGCATAAGTCATCGGTTCA	TGTCCTGAACCACTAGACGA	45.0	50.0	56.6	57.7	580-587 bp	1.0	68.8
trnT-GGU-trnE-UUC_p5	GTCATCAGTTCGAATCCGAGA	GCTGCCTCCTTGAAAGAGAG	47.6	55.0	57.6	58.3	526-533 bp	1.0	68.8