Hotspot

trnG-UCC-trnM-CAU

LSC · 13937-15897 · 1961 bp

Back to Dichanthelium

Pi 0.00395
MarkerSeek score 59.30
Primer available yes
Label trnG-UCC-trnM-CAU

Feature Metadata

label_name
trnG-UCC-trnM-CAU
feature_type
igs
region
LSC
length_bp
1961
variable_sites
20
pi
0.00395
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnM-CAU_p1 ACGAATCACACTTTTACCACT ACCACTAAGTGAAAAGCCCT 38.1 45.0 55.5 56.7 2046-2080 bp 1.0 43.1
trnG-UCC-trnM-CAU_p2 ACGAATCACACTTTTACCACT TGAACCGATGACTTATGCCT 38.1 45.0 55.5 56.6 2016-2050 bp 1.0 42.9
trnG-UCC-trnM-CAU_p3 ACGAATCACACTTTTACCACT CCGATGACTTATGCCTTACCA 38.1 47.6 55.5 57.5 2012-2046 bp 1.0 42.2
trnG-UCC-trnM-CAU_p4 ACGAATCACACTTTTACCACT ACCGATGACTTATGCCTTACC 38.1 47.6 55.5 57.5 2013-2047 bp 1.0 42.2
trnG-UCC-trnM-CAU_p5 GAACGAATCACACTTTTACCACT ACCACTAAGTGAAAAGCCCT 39.1 45.0 57.3 56.7 2048-2082 bp 1.0 42.2