Hotspot

ndhF

SSC · 127904-130156 · 2253 bp

Back to Diarthron

Pi 0.0337

MarkerSeek score 62.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2253
variable_sites: 76
pi: 0.0337
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TAGGAATAGGCGATGGGGAA	TGAAAAAGCTTTCAAGGCGG	50.0	45.0	57.6	57.5	2875 bp	0.5	52.7
ndhF_p2	AGGAATAGGCGATGGGGAAA	TGAAAAAGCTTTCAAGGCGG	50.0	45.0	58.5	57.5	2874 bp	0.5	52.1
ndhF_p3	GGAATAGGCGATGGGGAAAT	TGAAAAAGCTTTCAAGGCGG	50.0	45.0	57.4	57.5	2873 bp	0.5	51.2
ndhF_p4	ATAGGAATAGGCGATGGGGA	TGAAAAAGCTTTCAAGGCGG	50.0	45.0	57.4	57.5	2876 bp	0.5	50.8
ndhF_p5	TAGGAATAGGCGATGGGGAA	GAAAAAGCTTTCAAGGCGGC	50.0	50.0	57.6	58.9	2874 bp	0.5	49.8