Hotspot

ndhF-trnL-UAG

IRa · 130157-131854 · 1698 bp

Back to Diarthron

Pi 0.0336

MarkerSeek score 67.90

Primer available yes

Label ndhF-trnL-UAG

Feature Metadata

label_name: ndhF-trnL-UAG
feature_type: igs
region: IRa
length_bp: 1698
variable_sites: 54
pi: 0.0336
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-trnL-UAG_p1	ACACATTCCAACCAGTTCCC	CAGAAAAGGGCGGCTATCAT	50.0	50.0	58.0	58.0	2463-2524 bp	1.0	85.0
ndhF-trnL-UAG_p2	CACATTCCAACCAGTTCCCA	CAGAAAAGGGCGGCTATCAT	50.0	50.0	58.0	58.0	2462-2523 bp	1.0	85.0
ndhF-trnL-UAG_p3	ACACATTCCAACCAGTTCCC	ATGCCCCGAATAAGTCCAAC	50.0	50.0	58.0	57.9	2506-2567 bp	1.0	84.8
ndhF-trnL-UAG_p4	CACATTCCAACCAGTTCCCA	ATGCCCCGAATAAGTCCAAC	50.0	50.0	58.0	57.9	2505-2566 bp	1.0	84.8
ndhF-trnL-UAG_p5	ACACATTCCAACCAGTTCCC	CCCCGAATAAGTCCAACGAG	50.0	55.0	58.0	58.1	2503-2564 bp	1.0	84.7