Hotspot

rpl33-rps18

LSC · 70515-70845 · 331 bp

Back to Diarthron

Pi 0.035

MarkerSeek score 68.50

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 331
variable_sites: 11
pi: 0.035
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	CAAACATACAACTCACGGGGA	TCAATTCGATCCCCCGATTG	47.6	50.0	58.2	57.7	430-442 bp	1.0	73.3
rpl33-rps18_p2	AAACATACAACTCACGGGGA	TCAATTCGATCCCCCGATTG	45.0	50.0	56.8	57.7	429-441 bp	1.0	73.2
rpl33-rps18_p3	CAAACATACAACTCACGGGG	TCAATTCGATCCCCCGATTG	50.0	50.0	56.7	57.7	430-442 bp	1.0	72.9
rpl33-rps18_p4	CCAGTCGATTGGAATTGAGGA	TCAATTCGATCCCCCGATTG	47.6	50.0	57.7	57.7	470-482 bp	1.0	72.5
rpl33-rps18_p5	ACAAACATACAACTCACGGGG	TCAATTCGATCCCCCGATTG	47.6	50.0	58.5	57.7	431-443 bp	1.0	71.1