Hotspot

psaI-ycf4

LSC · 62638-63051 · 414 bp

Back to Diarthron

Pi 0.0387

MarkerSeek score 69.10

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 414
variable_sites: 16
pi: 0.0387
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	ATTTCCGGCAATTGCAATGG	TTGTTTTTCTAGACCCCGCT	45.0	45.0	58.0	57.1	533-540 bp	1.0	74.8
psaI-ycf4_p2	GGCAATTGCAATGGCTTCTT	TTGTTTTTCTAGACCCCGCT	45.0	45.0	57.9	57.1	527-534 bp	1.0	74.2
psaI-ycf4_p3	GGCCTAGTATTTCCGGCAAT	TTGTTTTTCTAGACCCCGCT	50.0	45.0	57.7	57.1	541-548 bp	1.0	72.8
psaI-ycf4_p4	ATTTCCGGCAATTGCAATGG	AAGGATAAAAGCCCAGCAGA	45.0	45.0	58.0	56.8	559-566 bp	1.0	72.6
psaI-ycf4_p5	ATTTCCGGCAATTGCAATGG	AGGATAAAAGCCCAGCAGAA	45.0	45.0	58.0	56.8	558-565 bp	1.0	72.6