Hotspot

trnF-GAA-ndhJ

LSC · 51299-51758 · 460 bp

Back to Diarthron

Pi 0.0286

MarkerSeek score 65.30

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 460
variable_sites: 13
pi: 0.0286
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	CATCCACGACTGAAGCGTAT	50.0	50.0	58.1	57.8	599-600 bp	1.0	80.4
trnF-GAA-ndhJ_p2	TGGTAATGGTCGGGATAGCT	CATCCACGACTGAAGCGTAT	50.0	50.0	57.9	57.8	646-647 bp	1.0	79.9
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	CCACGACTGAAGCGTATCTT	50.0	50.0	58.1	57.7	596-597 bp	1.0	79.9
trnF-GAA-ndhJ_p4	TGGTAATGGTCGGGATAGCT	CCACGACTGAAGCGTATCTT	50.0	50.0	57.9	57.7	643-644 bp	1.0	79.3
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	AATCATCCACGACTGAAGCG	50.0	50.0	58.1	58.4	602-603 bp	1.0	79.3