Pi
0.0306
MarkerSeek score
67.70
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 762-971 bp | 1.0 | 83.2 |
| trnT-UGU-trnL-UAA_p2 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 770-979 bp | 1.0 | 82.7 |
| trnT-UGU-trnL-UAA_p3 | GCGATGCTCTAACCTCTGAG | AGTCCGTAGCGTCTACCAAT | 55.0 | 50.0 | 57.9 | 58.2 | 776-985 bp | 1.0 | 81.3 |
| trnT-UGU-trnL-UAA_p4 | CTAACCTCTGAGCTAAGCGG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.5 | 58.0 | 754-963 bp | 1.0 | 80.3 |
| trnT-UGU-trnL-UAA_p5 | ATGCGATGCTCTAACCTCTG | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.5 | 58.0 | 764-973 bp | 1.0 | 80.1 |