Hotspot

psbC-trnS-UGA

LSC · 37281-37558 · 278 bp

Back to Diarthron

Pi 0.0323

MarkerSeek score 72.90

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 278
variable_sites: 8
pi: 0.0323
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	TCGTGGGTCATTTATGGCAC	GAGAGATGGCTGAGTGGTTG	50.0	55.0	58.3	58.0	446-476 bp	1.0	83.2
psbC-trnS-UGA_p2	GCAGCGGGATTTGAAAAAGG	GAGAGATGGCTGAGTGGTTG	50.0	55.0	58.3	58.0	405-435 bp	1.0	83.1
psbC-trnS-UGA_p3	TCGTGGGTCATTTATGGCAC	GCTGAGTGGTTGATAGCTCC	50.0	55.0	58.3	58.1	438-468 bp	1.0	82.9
psbC-trnS-UGA_p4	TCGTGGGTCATTTATGGCAC	GGCTGAGTGGTTGATAGCTC	50.0	55.0	58.3	58.1	439-469 bp	1.0	82.9
psbC-trnS-UGA_p5	TCGTGGGTCATTTATGGCAC	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.3	58.1	431-461 bp	1.0	82.9