Hotspot

atpH-atpI

LSC · 14678-15845 · 1168 bp

Back to Diarthron

Pi 0.0288

MarkerSeek score 73.10

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1168
variable_sites: 33
pi: 0.0288
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	GCAAGTCCTACAGCTAACCC	TTTTGCAACTTTAGCTGCGG	55.0	45.0	58.0	57.9	1282-1317 bp	1.0	82.2
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1255-1290 bp	1.0	82.2
atpH-atpI_p3	AGCGGCAGAAATCAATGGAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	57.9	57.9	1247-1282 bp	1.0	82.0
atpH-atpI_p4	GCAAGTCCTACAGCTAACCC	CGAATCCATGGAGGGTCATC	55.0	55.0	58.0	57.8	1252-1287 bp	1.0	82.0
atpH-atpI_p5	ATAACGGAAGCGGCAGAAAT	CGAATCCATGGAGGGTCATC	45.0	55.0	58.0	57.8	1225-1260 bp	1.0	82.0