Hotspot

atpF-atpH

LSC · 13921-14431 · 511 bp

Back to Diarthron

Pi 0.0407

MarkerSeek score 72.50

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 511
variable_sites: 20
pi: 0.0407
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CAGTGACCCAAGGAAACGAA	GGTTGTAGCATTGGCCCTTT	50.0	50.0	58.0	58.7	592-595 bp	1.0	79.2
atpF-atpH_p2	CAGTGACCCAAGGAAACGAA	AGCATTGGCCCTTTTATTTGC	50.0	42.9	58.0	58.0	586-589 bp	1.0	77.1
atpF-atpH_p3	CAGTGACCCAAGGAAACGAA	GGTTGTAGCATTGGCCCTT	50.0	52.6	58.0	58.0	592-595 bp	1.0	76.9
atpF-atpH_p4	CAGTGACCCAAGGAAACGAA	GCCCTTTTATTTGCCAATCCC	50.0	47.6	58.0	58.1	579-582 bp	1.0	76.6
atpF-atpH_p5	CAGTGACCCAAGGAAACGAA	GGCCCTTTTATTTGCCAATCC	50.0	47.6	58.0	58.1	580-583 bp	1.0	76.5