Hotspot

psbI-trnS-GCU

LSC · 8722-8806 · 85 bp

Back to Diarthron

Pi 0.0952

MarkerSeek score 72.90

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 85
variable_sites: 8
pi: 0.0952
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	CAAGCCGCTGTAAGTTTTCG	TGGACTAAAGCGTCGGATTG	50.0	50.0	58.0	58.0	669-737 bp	1.0	86.3
psbI-trnS-GCU_p2	ACTTAGACTTGGAGTCCCCC	TGGACTAAAGCGTCGGATTG	55.0	50.0	58.0	58.0	504 bp	0.5	59.6
psbI-trnS-GCU_p3	ACTTAGACTTGGAGTCCCCC	GTGGACTAAAGCGTCGGATT	55.0	50.0	58.0	58.0	505 bp	0.5	59.6
psbI-trnS-GCU_p4	CTTAGACTTGGAGTCCCCCA	TGGACTAAAGCGTCGGATTG	55.0	50.0	58.0	58.0	503 bp	0.5	59.6
psbI-trnS-GCU_p5	CTTAGACTTGGAGTCCCCCA	GTGGACTAAAGCGTCGGATT	55.0	50.0	58.0	58.0	504 bp	0.5	59.6