Hotspot

psbK-psbI

LSC · 8167-8610 · 444 bp

Back to Diarthron

Pi 0.0375

MarkerSeek score 70.70

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 444
variable_sites: 14
pi: 0.0375
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CAAGCCGCTGTAAGTTTTCG	TCCGAAGATGAAGAGCGAAA	50.0	45.0	58.0	57.0	463-534 bp	1.0	77.6
psbK-psbI_p2	CAAGCCGCTGTAAGTTTTCG	CCGAAGATGAAGAGCGAAAC	50.0	50.0	58.0	56.9	462-533 bp	1.0	77.3
psbK-psbI_p3	AGCCGCTGTAAGTTTTCGAT	TCCGAAGATGAAGAGCGAAA	45.0	45.0	57.9	57.0	461-532 bp	1.0	77.3
psbK-psbI_p4	CAAGCCGCTGTAAGTTTTCG	GAATCCGAAGATGAAGAGCGA	50.0	47.6	58.0	57.9	466-537 bp	1.0	77.2
psbK-psbI_p5	AAGCCGCTGTAAGTTTTCGA	TCCGAAGATGAAGAGCGAAA	45.0	45.0	58.1	57.0	462-533 bp	1.0	77.0