Hotspot

rps16-trnQ-UUG

LSC · 6283-7549 · 1267 bp

Back to Diarthron

Pi 0.0393

MarkerSeek score 75.50

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1267
variable_sites: 47
pi: 0.0393
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	TTTGGTCCCGCTATTCGAAG	45.0	50.0	58.1	58.0	1342-1357 bp	1.0	85.5
rps16-trnQ-UUG_p2	CCACATCGCTTCAAACGAAG	TTTGGTCCCGCTATTCGAAG	50.0	50.0	57.8	58.0	1322-1337 bp	1.0	84.8
rps16-trnQ-UUG_p3	CAGCAAATCGACCATGTCCT	TTTGGTCCCGCTATTCGAAG	50.0	50.0	58.3	58.0	1364-1379 bp	1.0	84.5
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	GTTTTGGTCCCGCTATTCGA	45.0	50.0	58.1	58.3	1344-1359 bp	1.0	83.5
rps16-trnQ-UUG_p5	ACCACATCGCTTCAAACGAA	TTTGGTCCCGCTATTCGAAG	45.0	50.0	58.4	58.0	1323-1338 bp	1.0	83.4