Hotspot

trnH-GUG-psbA

LSC · 192-510 · 319 bp

Back to Diarthron

Pi 0.045

MarkerSeek score 69.00

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 319
variable_sites: 13
pi: 0.045
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	TGAAGCTCCATCTACAAATGG	50.0	42.9	57.9	55.7	373-381 bp	1.0	58.9
trnH-GUG-psbA_p2	ACTGCCTTAATCCACTTGGC	TGAAGCTCCATCTACAAATGG	50.0	42.9	58.2	55.7	374-382 bp	1.0	58.7
trnH-GUG-psbA_p3	AATCCACTTGGCTACATCCG	TGAAGCTCCATCTACAAATGG	50.0	42.9	57.7	55.7	366-374 bp	1.0	57.4
trnH-GUG-psbA_p4	CTGCCTTAATCCACTTGGCT	TTGAAGCTCCATCTACAAATGG	50.0	40.9	57.9	56.4	374-382 bp	1.0	56.9
trnH-GUG-psbA_p5	ACTGCCTTAATCCACTTGGC	TTGAAGCTCCATCTACAAATGG	50.0	40.9	58.2	56.4	375-383 bp	1.0	56.7