Hotspot

ycf2

IRb · 88337-95287 · 6951 bp

Back to Dianella

Pi 0.00144

MarkerSeek score 44.20

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: IRb
length_bp: 6951
variable_sites: 10
pi: 0.00144
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	GGGGATACACGTTACACCAC	TGCTAGCAAATACCGCTGTT	55.0	45.0	58.0	57.9	330 bp	1.0	80.7
ycf2_p2	GGGGATACACGTTACACCAC	TGTTGCTAGCAAATACCGCT	55.0	45.0	58.0	57.9	333 bp	1.0	80.7
ycf2_p3	GGGGATACACGTTACACCAC	TTGCTAGCAAATACCGCTGT	55.0	45.0	58.0	57.9	331 bp	1.0	80.7
ycf2_p4	GGGGATACACGTTACACCAC	TTCCAAATCATTCCCGCAGT	55.0	45.0	58.0	57.8	296 bp	1.0	80.1
ycf2_p5	GGAACAAAGGGGGATACACG	TGCTAGCAAATACCGCTGTT	55.0	45.0	58.3	57.9	339 bp	1.0	78.8