Hotspot

rps4

LSC · 46215-46820 · 606 bp

Back to Dianella

Pi 0.0033

MarkerSeek score 60.60

Primer available yes

Label rps4

Feature Metadata

label_name: rps4
feature_type: gene
region: LSC
length_bp: 606
variable_sites: 2
pi: 0.0033
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4_p1	TGTCTGAACCCCCTTCTTTG	AGTGGATTTTTCTTTGCTTTGT	50.0	31.8	57.3	55.3	737 bp	1.0	40.2
rps4_p2	GTCTGAACCCCCTTCTTTGT	AGTGGATTTTTCTTTGCTTTGT	50.0	31.8	57.3	55.3	736 bp	1.0	40.2
rps4_p3	TGTCTGAACCCCCTTCTTTG	AGTGGATTTTTCTTTGCTTTGTT	50.0	30.4	57.3	56.0	737 bp	1.0	40.2
rps4_p4	GTCTGAACCCCCTTCTTTGT	AGTGGATTTTTCTTTGCTTTGTT	50.0	30.4	57.3	56.0	736 bp	1.0	40.2
rps4_p5	GTCTGAACCCCCTTCTTTGTT	AGTGGATTTTTCTTTGCTTTGT	47.6	31.8	58.1	55.3	736 bp	1.0	40.2