Hotspot

ndhI

SSC · 121274-121816 · 543 bp

Back to Dianella

Pi 0.00184

MarkerSeek score 49.80

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 543
variable_sites: 1
pi: 0.00184
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	TCTCAAACAAGAGAAAGAAACATCA	TGTAGTTTCGGACCAACCAA	32.0	45.0	57.1	57.0	653 bp	1.0	40.1
ndhI_p2	TCTCAAACAAGAGAAAGAAACATCA	TTGTAGTTTCGGACCAACCA	32.0	45.0	57.1	57.0	654 bp	1.0	40.1
ndhI_p3	ACCTATCTCAAACAAGAGAAAGA	TGTAGTTTCGGACCAACCAA	34.8	45.0	55.0	57.0	658 bp	1.0	40.1
ndhI_p4	ACCTATCTCAAACAAGAGAAAGA	TTGTAGTTTCGGACCAACCA	34.8	45.0	55.0	57.0	659 bp	1.0	40.1
ndhI_p5	TCTCAAACAAGAGAAAGAAACATCA	TGTAGTTTCGGACCAACCAAA	32.0	42.9	57.1	57.7	653 bp	1.0	40.1