Hotspot

ccsA

SSC · 125658-126668 · 1011 bp

Back to Dianella

Pi 0.00297

MarkerSeek score 46.50

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 1011
variable_sites: 3
pi: 0.00297
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TTCTCGATGGCTTCTCTTGC	TAGACACGCTGCTCTTAGGA	50.0	50.0	58.0	57.9	1721 bp	1.0	79.3
ccsA_p2	TTCTCGATGGCTTCTCTTGC	CGAGTGGCGGCATATTCTAA	50.0	50.0	58.0	57.9	1672 bp	1.0	79.1
ccsA_p3	CTCGATGGCTTCTCTTGCAT	TAGACACGCTGCTCTTAGGA	50.0	50.0	58.0	57.9	1719 bp	1.0	79.0
ccsA_p4	CTCGATGGCTTCTCTTGCAT	CGAGTGGCGGCATATTCTAA	50.0	50.0	58.0	57.9	1670 bp	1.0	78.8
ccsA_p5	TTCTCGATGGCTTCTCTTGC	GTAGACACGCTGCTCTTAGG	50.0	55.0	58.0	57.8	1722 bp	1.0	78.7