Hotspot

ccsA-trnL

SSC · 126669-126703 · 35 bp

Back to Dianella

Pi 0.0286

MarkerSeek score 61.80

Primer available yes

Label ccsA-trnL

Feature Metadata

label_name: ccsA-trnL
feature_type: igs
region: SSC
length_bp: 35
variable_sites: 1
pi: 0.0286
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-trnL_p1	GGGGGTCCCATTACAGAATT	TAGACACGCTGCTCTTAGGA	50.0	50.0	56.9	57.9	137 bp	1.0	70.4
ccsA-trnL_p2	GGGGGTCCCATTACAGAATT	GGTAGACACGCTGCTCTTAG	50.0	55.0	56.9	57.8	139 bp	1.0	69.8
ccsA-trnL_p3	GGGGGTCCCATTACAGAATT	GTAGACACGCTGCTCTTAGG	50.0	55.0	56.9	57.8	138 bp	1.0	69.8
ccsA-trnL_p4	GGGGGTCCCATTACAGAATT	CAAGTAAGCCGCTATGGTGA	50.0	50.0	56.9	57.7	163 bp	1.0	68.9
ccsA-trnL_p5	GGGGGTCCCATTACAGAATT	TCAAGTAAGCCGCTATGGTG	50.0	50.0	56.9	57.7	164 bp	1.0	68.8