Pi
0.0137
MarkerSeek score
61.70
Primer available
yes
Label
ndhI-ndhG
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| ndhI-ndhG_p1 | TGGACAAAGATAAACCCCTTGA | TGTATTGTTCCAGGTAAATCCA | 40.9 | 36.4 | 57.3 | 55.1 | 435-436 bp | 1.0 | 40.9 |
| ndhI-ndhG_p2 | TGGACAAAGATAAACCCCTTGA | CATGTATTGTTCCAGGTAAATCCA | 40.9 | 37.5 | 57.3 | 57.0 | 437-438 bp | 1.0 | 40.9 |
| ndhI-ndhG_p3 | TGGACAAAGATAAACCCCTTGA | TCCCAAAGAAAATGCAGAAT | 40.9 | 35.0 | 57.3 | 53.3 | 532-533 bp | 1.0 | 40.8 |
| ndhI-ndhG_p4 | TGGACAAAGATAAACCCCTTGA | ACTACTCCTAGACCTCCTAA | 40.9 | 45.0 | 57.3 | 52.9 | 492-493 bp | 1.0 | 40.8 |
| ndhI-ndhG_p5 | TGGACAAAGATAAACCCCTTG | TCCCAAAGAAAATGCAGAAT | 42.9 | 35.0 | 55.8 | 53.3 | 532-533 bp | 1.0 | 40.8 |