Hotspot

petA-psbJ

LSC · 64841-65682 · 842 bp

Back to Dianella

Pi 0.0095

MarkerSeek score 64.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 842
variable_sites: 8
pi: 0.0095
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCATCCGTTATTTTGGCAC	GGTTCATATTCCGGGTTGGG	50.0	55.0	58.1	58.3	960-961 bp	1.0	77.5
petA-psbJ_p2	GGCATCCGTTATTTTGGCAC	CATATTCCGGGTTGGGTTCA	50.0	50.0	58.1	57.6	956-957 bp	1.0	76.6
petA-psbJ_p3	GGCATCCGTTATTTTGGCAC	GTTCATATTCCGGGTTGGGT	50.0	50.0	58.1	57.6	959-960 bp	1.0	76.6
petA-psbJ_p4	TGTTCTTCTTGGCATCCGTT	GGTTCATATTCCGGGTTGGG	45.0	55.0	57.7	58.3	970-971 bp	1.0	76.0
petA-psbJ_p5	TTGTTCTTCTTGGCATCCGT	GGTTCATATTCCGGGTTGGG	45.0	55.0	57.7	58.3	971-972 bp	1.0	76.0