Hotspot

ndhC-trnV

LSC · 51434-53292 · 1859 bp

Back to Dianella

Pi 0.00541

MarkerSeek score 52.40

Primer available yes

Label ndhC-trnV

Feature Metadata

label_name: ndhC-trnV
feature_type: igs
region: LSC
length_bp: 1859
variable_sites: 10
pi: 0.00541
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV_p1	TGCCAAGATAGGAATAAAACCTGA	AGAAGGTCTACGGTTCGAGT	37.5	50.0	57.9	57.8	1964-1981 bp	1.0	46.7
ndhC-trnV_p2	TGCCAAGATAGGAATAAAACCTGA	AGCCCAATGCAACAAAATGC	37.5	45.0	57.9	58.5	2046-2063 bp	1.0	44.5
ndhC-trnV_p3	TGCCAAGATAGGAATAAAACCTGA	GAAGGTCTACGGTTCGAGTC	37.5	55.0	57.9	57.5	1963-1980 bp	1.0	44.0
ndhC-trnV_p4	TGCCAAGATAGGAATAAAACCTG	AGAAGGTCTACGGTTCGAGT	39.1	50.0	56.6	57.8	1964-1981 bp	1.0	43.9
ndhC-trnV_p5	TGCCAAGATAGGAATAAAACCTGA	GTTCGAGTCCGTATAGCCCT	37.5	55.0	57.9	58.7	1952-1969 bp	1.0	42.8