Hotspot

psaA-ycf3

LSC · 42601-43257 · 657 bp

Back to Dianella

Pi 0.00304

MarkerSeek score 60.50

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 657
variable_sites: 2
pi: 0.00304
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGATGT	GGAGGCTTGGTCTGATCAAG	50.0	55.0	58.0	58.0	849 bp	1.0	79.6
psaA-ycf3_p2	GCCCATTCCTCGAAAGATGT	CTTGGTCTGATCAAGCTGCT	50.0	50.0	58.0	57.9	844 bp	1.0	78.9
psaA-ycf3_p3	GCCCATTCCTCGAAAGATGT	TTGAAGATCACGAGGCGTTT	50.0	45.0	58.0	57.8	764 bp	1.0	78.5
psaA-ycf3_p4	GCCCATTCCTCGAAAGATGT	TTGGTCTGATCAAGCTGCTG	50.0	50.0	58.0	58.2	843 bp	1.0	78.4
psaA-ycf3_p5	GCCCATTCCTCGAAAGATGT	TTGGTTGAAGATCACGAGGC	50.0	50.0	58.0	58.2	768 bp	1.0	78.3