Hotspot

trnG-trnfM

LSC · 37285-37452 · 168 bp

Back to Dianella

Pi 0.0179

MarkerSeek score 62.80

Primer available yes

Label trnG-trnfM

Feature Metadata

label_name: trnG-trnfM
feature_type: igs
region: LSC
length_bp: 168
variable_sites: 3
pi: 0.0179
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-trnfM_p1	AGAAGATGCGGGTTCGATTC	GTTCAAATCCTGTCTCCGCA	50.0	50.0	58.1	58.2	221-223 bp	1.0	79.1
trnG-trnfM_p2	GAGAAGATGCGGGTTCGATT	GTTCAAATCCTGTCTCCGCA	50.0	50.0	58.1	58.2	222-224 bp	1.0	79.1
trnG-trnfM_p3	AGAAGATGCGGGTTCGATTC	GTAGCTCGCAAGGCTCATAA	50.0	50.0	58.1	57.8	255-257 bp	1.0	78.7
trnG-trnfM_p4	AGAAGATGCGGGTTCGATTC	TAGCTCGCAAGGCTCATAAC	50.0	50.0	58.1	57.8	254-256 bp	1.0	78.7
trnG-trnfM_p5	GAGAAGATGCGGGTTCGATT	TAGCTCGCAAGGCTCATAAC	50.0	50.0	58.1	57.8	255-257 bp	1.0	78.7