Hotspot

trnC-petN

LSC · 28455-29176 · 722 bp

Back to Dianella

Pi 0.00554

MarkerSeek score 61.90

Primer available yes

Label trnC-petN

Feature Metadata

label_name: trnC-petN
feature_type: igs
region: LSC
length_bp: 722
variable_sites: 4
pi: 0.00554
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-petN_p1	GGGGGACTGCAAATCCTTTA	AGTCCACTCCTTCCCCATAC	50.0	55.0	57.5	58.1	855-857 bp	1.0	75.2
trnC-petN_p2	GGGGGACTGCAAATCCTTTA	GTCCACTCCTTCCCCATACT	50.0	55.0	57.5	58.1	854-856 bp	1.0	75.2
trnC-petN_p3	GGGGGACTGCAAATCCTTTA	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.5	58.2	796-798 bp	1.0	75.0
trnC-petN_p4	GGGGGACTGCAAATCCTTTA	GAGTCCACTCCTTCCCCATA	50.0	55.0	57.5	57.8	856-858 bp	1.0	74.8
trnC-petN_p5	GGGGGACTGCAAATCCTTTA	CTAGAGTCCACTCCTTCCCC	50.0	60.0	57.5	58.2	859-861 bp	1.0	74.4