Hotspot

rpoB-trnC

LSC · 27110-28383 · 1274 bp

Back to Dianella

Pi 0.00715

MarkerSeek score 60.20

Primer available yes

Label rpoB-trnC

Feature Metadata

label_name: rpoB-trnC
feature_type: igs
region: LSC
length_bp: 1274
variable_sites: 9
pi: 0.00715
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC_p1	AACCTACAGAACCCCTCGAA	TAAAGGATTTGCAGTCCCCC	50.0	50.0	58.0	57.5	1395 bp	1.0	77.0
rpoB-trnC_p2	AATTCTTCCGACAAGCCCTG	TAAAGGATTTGCAGTCCCCC	50.0	50.0	58.2	57.5	1422 bp	1.0	75.8
rpoB-trnC_p3	ACCTACAGAACCCCTCGAAT	TAAAGGATTTGCAGTCCCCC	50.0	50.0	57.8	57.5	1394 bp	1.0	75.2
rpoB-trnC_p4	AACCTACAGAACCCCTCGAA	TTTGTTGATCAGGCGACACC	50.0	50.0	58.0	58.8	1432 bp	1.0	75.1
rpoB-trnC_p5	AACCTACAGAACCCCTCGAA	GACACCCGGATTTGAACTGG	50.0	55.0	58.0	58.8	1418 bp	1.0	74.5