Hotspot

atpH-atpI

LSC · 14390-14881 · 492 bp

Back to Dianella

Pi 0.00611

MarkerSeek score 60.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 492
variable_sites: 3
pi: 0.00611
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	ATAGGGGAATCCATGGAGGG	45.0	55.0	58.0	57.9	554-556 bp	1.0	79.7
atpH-atpI_p2	CGCAATACCTTCTACGGCTT	ATAGGGGAATCCATGGAGGG	50.0	55.0	58.1	57.9	642-644 bp	1.0	79.5
atpH-atpI_p3	TACCTTGACCAACTCCAGGT	ATAGGGGAATCCATGGAGGG	50.0	55.0	57.9	57.9	610-612 bp	1.0	79.1
atpH-atpI_p4	AACGGAAGCGGCAGAAATAA	ATAGGGGAATCCATGGAGGG	45.0	55.0	58.2	57.9	552-554 bp	1.0	78.6
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	ATAGGGGAATCCATGGAGGG	45.0	55.0	58.2	57.9	555-557 bp	1.0	78.6